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KMID : 0191119990140020165
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Journal of Korean Medical Science
1999 Volume.14 No. 2 p.165 ~ p.170
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Monitoring antibody titers to recombinant Core-NS3 fusion polypeptide is useful for evaluating hepatitis C virus infection and responses to interferon-alpha therapy
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Park YM
Byun BH/Choi JY/Bae SH/Kim BS/So HS/Ryu WS
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Abstract
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To evaluate the clinical feasibility of the antibody titer against a chimeric polypeptide (named Core 518),
in which a domain of Core and NS3 of hepatitis C virus (HCV) was fused, ELISA was performed in a total of 76
serum samples. Each serum was serially diluted using two-fold dilution method with distilled water into 10
concentrations. They were all positive for second generation anti-HCV assay (HCV EIA II; Abbott Laboratories).
Genotyping RT-PCR, quantitative competitive RT-PCR, and RIBA (Lucky Confirm; LG Biotech) were also assayed.
Anti-Core 518 antibody was detected in x 12800 or higher dilutions of sera from 35 of 43 chronic hepatitis C
(81.4%) and nine of 16 hepatocellular carcinoma sera (56.3%), one of four cirrhosis (25%), 0 of four acute
hepatitis C, and one of nine healthy isolated anti-HCV-positive subjects (p=0.0000). The anti-Core 518
antibody titers were well correlated with the presence of HCV RNA in serum (p=0.002). The anti-Core 518
antibody titers decreased significantly in nine of ten responders to IFN-alpha treatment. Monitoring anti-Core
518 titers may be helpful not only for differentiating the status of HCV infection among patients with various
type C viral liver diseases, but also for predicting responses to IFN-alpha treatment.
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